Submerged Fermentation of Animal Fat By-Products by Oleaginous Filamentous Fungi for the Production of Unsaturated Single Cell Oil

Animal waste fats were explored as a fermentation substrate for the production of high-value unsaturated single cell oil (SCO) using oleaginous fungi, Mucor circinelloides and Mortierella alpina. Both strains showed good growth lipid accumulation when animal fat carbon source. The biomass concentration 16.7 ± 2.2 gDCW/L content 54.1%wt (of dry weight) obtained in shake flask experiments, surpassing yield achieved batch fed-batch fermentation. In contrast, alpina gave highest (8.3 0.3 gDCW/L) (55.8%wt) Fat grown was able to produce arachidonic acid (ARA), ARA 23.8%wt total Gamma-linolenic (GLA) produced by both fungal strains. At end fermentation, GLA yields 4.51%wt 2.77%wt weight), respectively. This study demonstrates SCO-rich from